R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Abi1 (D3G6C) Rabbit mAb #39444
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55-65 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Abi1 (D3G6C) Rabbit mAb recognizes endogenous levels of total Abi1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr118 of human Abi1 protein.
Background
Abi1, Abi2 and Abi3 are members of the Abl1 interactor family, which function as adaptor signaling molecules down stream of the receptor tyrosine kinase Ab1 (1-3). In addition to Abl, Abi1 has been shown to interact with the important signaling transducers WAVE and p85PI3K to regulate cytoskeletal and growth signaling (4,5). Along its sequences, Abi1 has multiple modules for carrying on these interactions. It has a WAVE binding domain, which allows it to interact with WAVE, a homeo-domain/PEST domain, which, when phosphorylated can acts as a docking site for SH2 binding, a PXXP sequence to interact with the SH3 domain of Abl, and a C-terminal SH3 domain for interaction with the proline rich region of Ab1 (4,5). Abl can phosphorylate Abi1 on Y213 (6), the phosphorylated sequence serves as a docking site for both the SH2 domain of Abl and the SH2 domain of p85PI3K (7). Another important phosphorylation site for Abi1 is Y435. Phosphorylation of Abi1 at Y435 promotes tumor cell adhesion and invasion (8).
- Shi, Y. et al. (1995) Genes Dev 9, 2583-97.
- Dai, Z. and Pendergast, A.M. (1995) Genes Dev 9, 2569-82.
- Miyazaki, K. et al. (2000) Biochim Biophys Acta 1493, 237-41.
- Kotula, L. (2012) FEBS Lett 586, 2790-4.
- Hossain, S. et al. (2012) Genes Cancer 3, 402-13.
- Xiong, X. et al. (2008) Biochim Biophys Acta 1783, 737-47.
- Dubielecka, P.M. et al. (2010) FEBS Lett 584, 3279-86.
- Steinestel, K. et al. (2014) Mol Cancer 13, 145.
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