R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ABHD6 (D3C8N) Rabbit mAb #97573
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 32 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ABHD6 (D3C8N) Rabbit mAb recognizes endogenous levels of total ABHD6 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile121 of human ABHD6 protein.
Background
ABHD6 (α/β-Hydrolase domain-containing 6) is a monoacylglycerol lipase, ubiquitously expressed with the highest expression in brown adipose tissue, small intestine, and brain (1). A high-fat diet upregulates ABHD6 mRNA expression in small intestine and liver, and ABHD6 knockdown protects against high-fat diet-induced obesity, hepatic steatosis, and systemic insulin resistance (2). In addition, it has been shown that ABHD6 is a negative modulator of glucose-stimulated insulin secretion (3). In the central nervous system, ABHD6 is expressed postsynptically and degrades the endocannabinoid 2-arachidonoylglycerol (2-AG), an endogenous activator of cannabinoid receptors (4,5). Inhibitors of α/β-hydrolase domain 6 (ABHD6) have been actively pursued as a promising approach to treat inflammation, metabolic disorders, and epilepsy (2,6,7).
- Lord, C.C. et al. (2013) Biochim Biophys Acta 1831, 792-802.
- Thomas, G. et al. (2013) Cell Rep 5, 508-20.
- Zhao, S. et al. (2014) Cell Metab 19, 993-1007.
- Blankman, J.L. et al. (2007) Chem Biol 14, 1347-56.
- Marrs, W.R. et al. (2010) Nat Neurosci 13, 951-7.
- Alhouayek, M. et al. (2013) Proc Natl Acad Sci U S A 110, 17558-63.
- Naydenov, A.V. et al. (2014) Neuron 83, 361-71.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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