R Recombinant
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ABCG2 (D5V2K) XP® Rabbit mAb #42078
Filter:
- WB
- IP
- IHC
Western blot analysis of extracts from RPMI 8226 and A549 cells and human placenta using ABCG2 (D5V2K) XP® Rabbit mAb.
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65-80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:175 - 1:700 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #61474.
For a carrier free (BSA and azide free) version of this product see product #61474.
Protocol
Specificity / Sensitivity
ABCG2 (D5V2K) XP® Rabbit mAb recognizes endogenous levels of total ABCG2 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human ABCG2 protein.
Background
ABCG2 (BCRP1/ABCP/MXR) is a member of the ATP-binding cassette transporter family that functions as ATP-dependent transporters for a wide variety of chemical compounds and are associated with drug-resistance in cancer cells (1-6). ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane spanning regions consistent with it functioning as a half-transporter. The ABC family can exist as either full-length transporters or as half-transporters that form functional transporters through homo- or heterodimerization. High expression of ABCG2 was found in placenta as well as cell lines selected for resistance to a number of chemotherapeutic drugs, including mitoxantrone, doxorubicin, topotecan and flavopiridol. In rodents, the highest expression of ABCG2 was found in kidney (8). ABCG2 expression has also been observed in stem cell populations, particularly in hematopoietic and neuronal stem cells and is downregulated with differentiation (9-11).
- Doyle, L.A. and Ross, D.D. (2003) Oncogene 22, 7340-58.
- Allen, J.D. et al. (1999) Cancer Res 59, 4237-41.
- Doyle, L.A. et al. (1998) Proc Natl Acad Sci U S A 95, 15665-70.
- Allikmets, R. et al. (1998) Cancer Res 58, 5337-9.
- Miyake, K. et al. (1999) Cancer Res 59, 8-13.
- Robey, R.W. et al. (2001) Clin Cancer Res 7, 145-52.
- Zhou, S. et al. (2001) Nat Med 7, 1028-34.
- Honscha, W. et al. (2000) Hepatology 31, 1296-304.
- Scharenberg, C.W. et al. (2002) Blood 99, 507-12.
- Islam, M.O. et al. (2005) Neurosci Res 52, 75-82.
- Bunting, K.D. (2002) Stem Cells 20, 11-20.
限制使用
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