Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ABCE1 (F3S8L) Rabbit mAb #95513
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 67 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ABCE1 (F3S8L) Rabbit mAb recognizes endogenous levels of total ABCE1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro575 of human ABCE1 protein.
Background
ATP-binding cassette sub-family E1 (ABCE1), also known as HP68 or RNase L inhibitor (RLI), was originally discovered to regulate RNase L, an endoribonuclease in the interferon (IFN) pathway (1,2). ABC transporters are involved in DNA repair, chromatin maintenance, and translation, though ABCE1 lacks transmembrane domains other members contain (3-5). ABCE1 is a highly conserved protein from archaea to eukaryotes and plays many essential roles in the RNA lifecycle. It is capable of binding initiation factors eIF2alpha and eIF5 to assist in mRNA translation (6). ABCE1 is also capable of binding release factors eRF1 and eRF3 to contribute to translation termination, and can additionally dissociate stalled and vacant ribosomes during translation (7-9). During HIV infection, the virus can utilize ABCE1 to help form viral capsids (10,11). Furthermore, ABCE1 also contributes to the silencing of RNA interference (12). ABCE1 protein expression is high in lung adenocarcinoma and contributes to Myc driven cancers (13,14).
- Bisbal, C. et al. (1995) J Biol Chem 270, 13308-17.
- Diriong, S. et al. (1996) Genomics 32, 488-90.
- Rees, D.C. et al. (2009) Nat Rev Mol Cell Biol 10, 218-27.
- Hopfner, K.P. and Tainer, J.A. (2003) Curr Opin Struct Biol 13, 249-55.
- Kerr, I.D. (2004) Biochem Biophys Res Commun 315, 166-73.
- Chen, Z.Q. et al. (2006) J Biol Chem 281, 7452-7.
- Khoshnevis, S. et al. (2010) EMBO Rep 11, 214-9.
- Pisareva, V.P. et al. (2011) EMBO J 30, 1804-17.
- Barthelme, D. et al. (2011) Proc Natl Acad Sci USA 108, 3228-33.
- Dooher, J.E. and Lingappa, J.R. (2004) J Virol 78, 1645-56.
- Lingappa, J.R. et al. (2006) J Biol Chem 281, 3773-84.
- Kärblane, K. et al. (2015) PLoS One 10, e0116702.
- Ren, Y. et al. (2012) Oncol Rep 27, 965-70.
- Gao, J. et al. (2020) Cancer Res 80, 3706-3718.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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