Render Target: SSR
Render Timestamp: 2024-11-21T22:15:00.020Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:18:58.589
Product last modified at: 2024-09-20T07:01:37.623Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

A2B5 Mouse mAb #8763

Filter:
  • IF

Inquiry Info. # 8763

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    Supporting Data

    REACTIVITY R
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgM
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • R-Rat 

    Product Information

    Product Usage Information

    It is strongly recommended that these directions be followed for staining of live, unpermeabilized cells when using this antibody. Dilute primary antibody 1:100 in culture medium containing 0.1% sodium azide*. Aspirate cell culture medium, add diluted antibody and incubate cells for 30 minutes at 37ºC. Aspirate antibody solution, rinse gently with pre-warmed PBS, and fix with methanol-free, 4% formaldehyde diluted in PBS for 15 minutes at 37ºC. Rinse cells with PBS 3 times for 5 minutes each. For best results, detect with a fluorochrome-conjugated anti-mouse IgM secondary antibody (per manufacturer's recommendation, diluted in PBS). Prior to imaging, rinse samples in PBS 3 times for 5 minutes each and mount in an anti-fade reagent (ProLong® Gold Antifade Reagent #9071).

    * sodium azide is included to prevent capping.

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    A2B5 Mouse mAb recognizes endogenous levels of A2B5 antigen. A2B5 Mouse mAb has been validated for use with the recommended staining protocol. Fixation of cells prior to staining will lead to artifacts. Based on literature publications, this antibody is predicted to cross-react with chicken, rat, bovine, and human samples.

    Species Reactivity:

    Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with embryonic chicken retinal cells.

    Background

    A2B5 Mouse mAb recognizes a cell surface ganglioside epitope that has been utilized as a marker for identification of various cell types. A2B5 Mouse mAb has been used to mark specific cell populations such as neuroendocrine cells, thymic epithelial cells (1), and glial precursors that give rise to type II astrocytes and oligodendrocytes (2-4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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