It is strongly recommended that these directions be followed for staining of live, unpermeabilized cells when using this antibody. Dilute primary antibody 1:100 in culture medium containing 0.1% sodium azide*. Aspirate cell culture medium, add diluted antibody and incubate cells for 30 minutes at 37ºC. Aspirate antibody solution, rinse gently with pre-warmed PBS, and fix with methanol-free, 4% formaldehyde diluted in PBS for 15 minutes at 37ºC. Rinse cells with PBS 3 times for 5 minutes each. For best results, detect with a fluorochrome-conjugated anti-mouse IgM secondary antibody (per manufacturer's recommendation, diluted in PBS). Prior to imaging, rinse samples in PBS 3 times for 5 minutes each and mount in an anti-fade reagent (ProLong^® Gold Antifade Reagent #9071).

* sodium azide is included to prevent capping.

Application	Dilution
Immunofluorescence (Immunocytochemistry)	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

A2B5 Mouse mAb recognizes endogenous levels of A2B5 antigen. A2B5 Mouse mAb has been validated for use with the recommended staining protocol. Fixation of cells prior to staining will lead to artifacts. Based on literature publications, this antibody is predicted to cross-react with chicken, rat, bovine, and human samples.

Species Reactivity:

Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with embryonic chicken retinal cells.

Background

A2B5 Mouse mAb recognizes a cell surface ganglioside epitope that has been utilized as a marker for identification of various cell types. A2B5 Mouse mAb has been used to mark specific cell populations such as neuroendocrine cells, thymic epithelial cells (1), and glial precursors that give rise to type II astrocytes and oligodendrocytes (2-4).

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

For Research Use Only. Not For Use In Diagnostic Procedures.

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A2B5 Mouse mAb #8763

Supporting Data

Application Key:

Species Cross-Reactivity Key:

Product Information

Product Usage Information