Cyclin Antibody Sampler Kit #9869
Product Information
Kit Usage Information
Protocols
- 2927: Western Blotting, Immunoprecipitation (Magnetic)
- 2936: Western Blotting, Immunohistochemistry (Paraffin)
- 2978: Western Blotting, Immunohistochemistry (Paraffin)
- 3741: Western Blotting, Immunoprecipitation (Agarose)
- 4129: Western Blotting
- 4132: Western Blotting
- 4138: Western Blotting, Immunofluorescence
- 4656: Western Blotting
- 7074: Western Blotting
- 7076: Western Blotting
Product Description
The Cyclin Antibody Sampler Kit provides an economical means of evaluating the presence of cyclin proteins in cells. The kit contains enough primary and secondary antibodies to perform two western blot experiments with each antibody.
Specificity / Sensitivity
Each antibody in the Cyclin Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other cyclin proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues within the carboxy terminus of cyclin D1, the amino terminus of cyclin D2, recombinant human cyclin E1, or cyclin A2. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues within the carboxy terminus of cyclin H, cyclin E2, or the amino terminus of cyclin B. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Background
Control of the cell cycle is regulated by a multitude of cellular events and processes. The cyclin dependent kinases (CDK) regulate many of these pathways and constitute an active complex when associated with their cyclin partners. This activity is controlled primarily by phosphorylation, which determines subcellular localization of the CDK/cyclin complex (1,2). Some phosphorylation events control the function of cytoplasmic retention sequences while other events regulate nuclear localization and export sequence function (3,4). Cyclin and cyclin-dependent kinase inhibitor (CKI) levels are regulated by ubiquitination and degradation via the ubiquitin proteasome pathway (5). A variety of CKI proteins associate with these complexes and modulate access to regulatory domains on cyclins (6). Additional complexity is generated as the controlled protein levels of each cyclin oscillate with the stages of cell cycle. Increased expression of Cyclin D1 is associated with certain types of cancer (7,8) and may associate with TSC2 (tuberin) independent of its Cdk partner (9).
- Krempler, A. et al. (2005) Cell Mol Life Sci 62, 1379-87.
- Toyoshima, F. et al. (1998) EMBO J 17, 2728-35.
- Porter, L.A. and Donoghue, D.J. (2003) Prog Cell Cycle Res 5, 335-47.
- Benzeno, S. et al. (2006) Oncogene 25, 6291-303.
- Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.
- Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
- Sherr, C.J. (1996) Science 274, 1672-7.
- Kim, J.K. and Diehl, J.A. (2009) J Cell Physiol 220, 292-6.
- Zacharek, S.J. et al. (2005) Cancer Res 65, 11354-60.
限制使用
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