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EOMES (E4Z4X) Rabbit mAb (ChIP Formulated) #66325
Filter:
- ChIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from NK-92 cells and either EOMES (E4Z4X) Rabbit mAb (ChIP Formulated) or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human IFN-γ Promoter Primers #13051, SimpleChIP® Human PRF1 Promoter Primers #9014, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
EOMES (E4Z4X) Rabbit mAb (ChIP Formulated) recognizes endogenous levels of total EOMES protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn654 of human EOMES protein.
Background
The T-box family of transcription factors is named for their shared homology with the DNA binding domain of the mouse brachyury (T) gene product. Members of this family bind DNA and are capable of transcriptional activation. They also have evolutionarily conserved expression patterns and roles in embryonic development, primarily mesoderm development (1). EOMES, or Tbr2 (T-box brain 2), is a master regulator of mesoderm formation that is also essential for trophoblast formation, gastrulation, neurogenesis, and the differentiation of certain T cell subsets. Embryos from EOMES knockout mice die soon after implantation due to their inability to develop a trophoblast (2,3). Conditional neural knockout mice show defects in development of a specific population of neural progenitors known as intermediate-stage progenitor cells (IPCs) that give rise only to neurons (4,5). These cells are formed from the radial glia in the ventricular and sub-ventricular zones of the cortex. Expression of EOMES increases as cells develop from radial glia to IPCs and then decreases as IPCs progress to neurons. Recent evidence suggests that EOMES and IPCs may also play a role in neurogenesis in the adult hippocampal SGZ (5). EOMES is also a key transcription factor for memory T cells and for full effector differentiation of CD8+ T cells (6). Expression of EOMES is induced in CD8+ T cells following viral infection and bacterial infection where sufficient IL-12 has been produced to elicit acute host cell response (7).
- Showell, C. et al. (2004) Dev Dyn 229, 201-18.
- Russ, A.P. et al. (2000) Nature 404, 95-9.
- Strumpf, D. et al. (2005) Development 132, 2093-102.
- Englund, C. et al. (2005) J Neurosci 25, 247-51.
- Hodge, R.D. et al. (2008) J Neurosci 28, 3707-17.
- Takayanagi, M. et al. (2003) Rheumatol Int 23, 315-8.
- Takemoto, N. et al. (2006) J Immunol 177, 7515-9.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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