R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb (BSA and Azide Free) #81977
Filter:
- WB
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75-120 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier free version of product #47213. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #47213
For standard formulation of this product see product #47213
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total 4F2hc/SLC3A2 protein. This antibody is predicted to detect multiple isoforms of 4F2hc/SLC3A2.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val509 of human 4F2hc/SLC3A2 protein.
Background
4F2hc is a transmembrane protein that belongs to the solute carrier family. 4F2hc forms heterodimeric complexes with various amino acid transporters such as LAT1 and LAT2 and regulates uptake of amino acids (1-5). 4F2hc is one of the earliest expressed antigens on the surface of activated human lymphocytes (6), hence it is also named CD98. 4F2hc is expressed in all cell types with the exception of platelets, and is expressed at highest levels in the tubules of the kidney and the gastrointestinal tract (7,8). It is localized at the plasma membrane when associated with LAT1 or LAT2 (9) and at the apical membrane of placenta (10). Research studies have shown that 4F2hc is highly expressed in various tumors including glioma (11), ovarian cancer (12), and astrocytomas (13), and it has been implicated in tumor progression and correlated with poor outcome in patients with pulmonary neuroendocrine tumors (14). 4F2hc is also involved in integrin trafficking through association with β1 and β4 integrins, and regulates keratinocyte adhesion and differentiation (15).
- Kanai, Y. et al. (1998) J Biol Chem 273, 23629-32.
- Mastroberardino, L. et al. (1998) Nature 395, 288-91.
- Pfeiffer, R. et al. (1999) EMBO J 18, 49-57.
- Pineda, M. et al. (1999) J Biol Chem 274, 19738-44.
- Sato, H. et al. (1999) J Biol Chem 274, 11455-8.
- Lumadue, J.A. et al. (1987) Proc Natl Acad Sci USA 84, 9204-8.
- Rossier, G. et al. (1999) J Biol Chem 274, 34948-54.
- Dave, M.H. et al. (2004) J Physiol 558, 597-610.
- Bröer, A. et al. (2001) Biochem J 355, 725-31.
- Ritchie, J.W. and Taylor, P.M. (2001) Biochem J 356, 719-25.
- Okubo, S. et al. (2010) J Neurooncol 99, 217-25.
- Kaji, M. et al. (2010) Int J Gynecol Cancer 20, 329-36.
- Nawashiro, H. et al. (2006) Int J Cancer 119, 484-92.
- Kaira, K. et al. (2011) Oncol Rep 26, 931-7.
- Lemaître, G. et al. (2011) J Dermatol Sci 61, 169-79.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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