4E-T Antibody #2297
Filter:
- WB
Western blot analysis of extracts from various cell lines, using 4E-T Antibody.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
4E-T Antibody detects endogenous levels of total 4E-T protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human 4E-T. Antibodies are purified by protein A and peptide affinity chromatography.
Background
During translation, eIF4E binds to the 5' cap structure of mRNA and helps mRNA bind to the ribosome. There are several factors that associate with eIF4E. One of these factors is eIF4E-Transporter (4E-T) (1). 4E-T has an eIF4E binding site, a nuclear localization signal and two nuclear export signals (1). Studies demonstrate that 4E-T functions as a nucleocytoplasmic shuttling protein mediating the import of eIF4E into the nucleus (1). Recent findings indicate that 4E-T, along with eIF4E, is concentrated in the processing bodies (P-bodies) in the cytoplasm (2). Furthermore, 4E-T decreases mRNA stability (2).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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