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MAGI3-Akt3 Fusion Protein (D4D3P) Rabbit mAb #48086
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- IP
- IF
Inquiry Info. # 48086
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Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 135 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:3200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MAGI3-Akt3 Fusion Protein (D4D3P) Rabbit mAb recognizes transfected MAGI3-Akt3 fusion protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the fusion site of MAGI3-Akt3 fusion protein.
Background
MAGI3-Akt3 fusion was first detected in triple-negative breast cancer tissues in a sequence analysis research study (1). Rearrangement occurs between the genes MAGI3 (membrane-associated guanylate kinase, WW and PDZ domain containing 3) on chromosome 1p and Akt3 on chromosome 1q, leading to a translocation from intron 9 in MAGI3 to intron1 of Akt3. This rearrangement yields an in-frame fusion gene that translates into a MAGI3-Akt3 fusion protein. Overexpression studies show that MAGI3-Akt3 fusion protein leads to the constitutive activation of Akt kinase (1). Further research studies demonstrate that this fusion is not a recurrent event in triple-negative breast cancer (2,3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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