FIP1L1 Antibody #3213
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Inquiry Info. # 3213
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Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 68-80 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:25 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
FIP1L1 Antibody detects the endogenous levels of total FIP1L1 proteins.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe164 of human FIP1L1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
FIP1L1 is the mammalian homolog of yeast Fip1p (factor interacting with poly(A) polymerase (PAP)). It is a subunit of cleavage and polyadenylation specificity factor (CPSF) that binds to PAP and to U-rich sequences on the pre-mRNA, stimulating polyadenylation of pre-mRNA (1). FIP1L1 has several splicing variants (1). FIP1L1 is fused to platelet-derived growth factor receptor α gene (PDGFRα) by an interstitial deletion on chromosome 4q12 in some patients with idiopathic hypereosinophilic syndrome (HES) and chronic eosinophilic leukemia (CEL). Τhe resulting FIP1L1-PDGFRα fusion protein has constitutively elevated tyrosine kinase activity (2).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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