R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb #54560
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70-120 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb recognizes endogenous levels of total 2B4/SLAMF4/CD244 protein. This antibody cross-reacts with an unidentified protein of 18 kDa in some cell extracts.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp367 of human 2B4/SLAMF4/CD244 protein.
Background
2B4, also known as signaling lymphocytic activation molecule family member 4 (SLAMF4) and cluster of differentiation 244 (CD244), is a heterophilic cell surface receptor expressed on a variety of immune cells, including natural killer (NK) cells, T cells, eosinophils, mast cells, and dendritic cells. 2B4 has been shown to have both immune stimulatory and inhibitory effects on cells (1-11). Upon engagement of its ligand CD48, 2B4 can enhance immune cell signaling, cytokine production, non-major histocompatibility complex-restricted cytotoxicity, and cell migration (1-8). Conversely, at early stages of NK cell differentiation, 2B4 may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (10). 2B4 also inhibits inflammatory responses in dendritic cells (11). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
- Speiser, D.E. et al. (2001) J Immunol 167, 6165-70.
- Nakajima, H. et al. (1999) Eur J Immunol 29, 1676-83.
- Valiante, N.M. and Trinchieri, G. (1993) J Exp Med 178, 1397-406.
- Munitz, A. et al. (2005) J Immunol 174, 110-8.
- Sivori, S. et al. (2000) Eur J Immunol 30, 787-93.
- El-Shazly, A.E. et al. Int J Immunopathol Pharmacol 24, 949-60.
- Chen, L. and Flies, D.B. (2013) Nat Rev Immunol 13, 227-42.
- Assarsson, E. et al. (2005) Mol Immunol 42, 419-23.
- Elishmereni, M. et al. (2014) J Invest Dermatol 134, 2928-37.
- Sivori, S. et al. (2002) Proc Natl Acad Sci U S A 99, 4526-31.
- Georgoudaki, A.M. et al. (2015) Immunol Cell Biol 93, 581-90.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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