CYP1A2 (D2V7S) Mouse mAb #14719
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CYP1A2 (D2V7S) Mouse mAb recognizes endogenous levels of total CYP1A2 protein. This antibody does not cross-react with CYP1A1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with 3-methylcholanthrene-induced rat liver microsomal fractions.
Background
Cytochrome P450 (CYP) is a family of enzymes that contain a heme group (1). These enzymes, when reduced and bound by carbon monoxide, maximally absorb light of 450 nm (1). Type I cytochrome P450s are found in mitochondria and function in the biosynthesis of essential molecules (1). Type II cytochrome P450s are found in endoplasmic reticulum (1). Some type II cytochrome P450s play a role in the biosynthesis of essential molecules whereas others metabolize xenobiotics (1). Research studies show that cytochrome P450s form various heteromeric complexes with other members of the P450 family influencing their catalytic activities (2-4). CYP1A2 is in the endoplasmic reticulum of hepatocytes and responsible for the breakdown of a variety of xenobiotic substances and bioactivation of carcinogens (2, 5). CYP1 enzymes, including CYP1A2, have been implicated in smoking-related osteoporosis (6). A meta-analysis shows that a particular polymorphism in CYP1A2 is potentially linked to increased cancer risk (5).
- Miller, W.L. (2012) Sci Signal 5, pt11.
- Backes, W.L. and Kelley, R.W. (2003) Pharmacol Ther 98, 221-33.
- Reed, J.R. et al. (2010) J Biol Chem 285, 8942-52.
- Kelley, R.W. et al. (2006) Biochemistry 45, 15807-16.
- Wang, H. et al. (2012) BMC Cancer 12, 528.
- Iqbal, J. et al. (2013) Proc Natl Acad Sci U S A 110, 11115-20.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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