InTraSeq™ 3' Assay Kit #82906
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Product Description
The InTraSeq™ method enables researchers to measure proteins (cytoplasmic, surface, and nuclear), including post-translational modifications (PTMs), along with RNA in their single-cell assays. While single-cell RNA sequencing (scRNAseq) has transformed the field of cellular and molecular biology, it only measures RNA transcripts within single cells. This presents a significant challenge, as RNA levels don't necessarily translate to protein abundance or activity. The InTraSeq technology addresses this limitation and can unravel missing information in scRNAseq experiments, uncovering new cell states and crucial biological data in single-cell analyses, especially since RNA does not reflect PTMs.
The InTraSeq™ 3’ Assay Kit provides important controls to ensure a successful experiment. The kit contains a negative control Rabbit (DA1E) mAb IgG XP® Isotype Control (InTraSeq™ 3' Conjugate 3000) #81472 conjugated to a 10x Genomics Feature Barcode, a positive control Histone H3 (D1H2) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3002) #68984 conjugated to a 10x Genomics Feature Barcode, and a protocol for performing 8 assays. The InTraSeq™ 3’ Assay Kit is specifically designed to be used with the InTraSeq™ 3' Antibody Cocktails and InTraSeq™ 3' Conjugates and is validated by CST using the 10x Genomics Chromium Single Cell 3' Kits with Feature Barcoding technology.
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