Posibeads™ G4S Linker Positive Control Beads #88651
Product Information
Product Description
Posibeads™ provide a unique positive control to confirm specific binding and fluorescence of your antibody conjugate of interest. Posibeads™ behave like cells in suspension, and can be carried through immunostaining and wash steps following the same procedure as your cell-based samples. The 5 nm non-fluorescent polystyrene beads are coated with G4S linker peptide. When incubated in the presence of a fluorescent antibody conjugate targeting G4S linker, the antibody will bind to the beads and result in increased fluorescence relative to unlabeled beads. Unlike compensation beads, the binding is driven by the antibody specifically targeting its antigen. Because of this, these Posibeads™ can be used as a specific control for a G4S linker antibody even when tested as part of a panel of other conjugated antibodies.
Posibeads™ are reproducibly generated and can also be used as a control to enable comparison of fluorescence across multiple instruments, sites, timepoints, and experiments. They can be used as a separate standalone sample or added to a sample of interest to serve as an internal positive control verifying the addition and functional activity of the conjugated antibody. Posibeads™ are not known to bind to any cell types, but a lower number of beads is recommended when mixing with cells to reduce the likelihood of acquiring concurrent or overlapping cell and bead events.
Posibeads™ are reproducibly generated and can also be used as a control to enable comparison of fluorescence across multiple instruments, sites, timepoints, and experiments. They can be used as a separate standalone sample or added to a sample of interest to serve as an internal positive control verifying the addition and functional activity of the conjugated antibody. Posibeads™ are not known to bind to any cell types, but a lower number of beads is recommended when mixing with cells to reduce the likelihood of acquiring concurrent or overlapping cell and bead events.
Product Usage Information
Posibeads™ can be used as an independent positive control sample or mixed with cells to function as an internal positive control. Recommended usage is 5 µL (around 100,000 beads) for use as an independent sample or 1 µL when mixed with cells. (If desired, perform a titration to determine the optimal ratio of beads to cells for your experiment.)
1. Prepare cells/tissue in tubes or wells as necessary for your experiment. If Posibeads™ will be used as an independent positive control, add an equivalent volume of buffer to empty tube(s) or well(s) as desired.
NOTE: Plan to include at least one unstained control to establish background fluorescence in your channel(s) of interest.
2. Mix or vortex the Posibeads™ immediately prior to use to ensure the beads are evenly distributed in solution.
3. Add desired volume of Posibeads™ to the control tube(s) or well(s), or add directly into the cell samples for use as an internal control.
4. Proceed with immunostaining following established protocols. The same incubation and wash steps should be performed with samples of interest and the Posibead control samples. The same centrifugation conditions may be used for both Posibeads™ and your cells of interest.
5. Acquire samples on flow cytometer. In mixed samples, beads will be differentiated from cells by high side scatter and low forward scatter.
1. Prepare cells/tissue in tubes or wells as necessary for your experiment. If Posibeads™ will be used as an independent positive control, add an equivalent volume of buffer to empty tube(s) or well(s) as desired.
NOTE: Plan to include at least one unstained control to establish background fluorescence in your channel(s) of interest.
2. Mix or vortex the Posibeads™ immediately prior to use to ensure the beads are evenly distributed in solution.
3. Add desired volume of Posibeads™ to the control tube(s) or well(s), or add directly into the cell samples for use as an internal control.
4. Proceed with immunostaining following established protocols. The same incubation and wash steps should be performed with samples of interest and the Posibead control samples. The same centrifugation conditions may be used for both Posibeads™ and your cells of interest.
5. Acquire samples on flow cytometer. In mixed samples, beads will be differentiated from cells by high side scatter and low forward scatter.
Storage
Store at 4 ºC. Supplied in 1X PBS (pH 7.4), 0.05% Tween 20, and 0.05% sodium azide.
限制使用
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