Mouse Naive/Effector/Memory T Cell Markers Flow Cytometry Panel #78148
Important Ordering Details
Custom Ordering Details: Product is assembled upon order. Please allow up to three business days for your product to be processed.Product Information
Protocol
Product Description
The Mouse Naive/Effector/Memory T Cell Markers Flow Cytometry Panel can be used to distinguish naive, effector, and memory mouse T cells in both CD4 and CD8 T cell populations.
T cells are identified by expression of CD3. There are two major subsets of conventional T cells: helper T cells which express CD4, and cytotoxic T cells which express CD8. Naive, effector, effector memory, and central memory T cell states can be distinguished by differential expression patterns of CD44, CD62L, and CD127/IL-7Rα.
T cells are identified by expression of CD3. There are two major subsets of conventional T cells: helper T cells which express CD4, and cytotoxic T cells which express CD8. Naive, effector, effector memory, and central memory T cell states can be distinguished by differential expression patterns of CD44, CD62L, and CD127/IL-7Rα.
Product Usage Information
All antibodies in this kit are compatible with the Flow Cytometry, Live Cell Protocol for Directly Conjugated Antibodies and can be used in a single staining mix. After antibody staining and prior to acquisition on a flow cytometer, we recommend adding a membrane impermeable viability dye such as Propidium Iodide or 7-AAD to enable identification and exclusion of dead cells from the analysis.
Gating strategy for observing naive, effector, and memory T cell subsets:
If Propidium Iodide or 7-AAD was used, first gate on viable cells. Next, gate on lymphocytes based on forward scatter and side scatter. Helper T cells are the CD3+CD4+ cells within the lymphocyte gate. Cytotoxic T cells are the CD3+CD8+ cells within the lymphocyte gate. Next, observe expression of CD44 vs. CD62L on either helper T cells or cytotoxic T cells. Naive T cells are CD44-CD62L+. Central memory T cells are CD44+CD62L+. Effector memory and effector cells are CD44+CD62L-. To distinguish effector memory and effector cells, observe expression of CD127/IL-7Rα on the CD44+CD62L- population. Effector memory cells are positive for expression of CD127/IL-7Rα. Effector cells are negative for expression of CD127/IL-7Rα. Naive and central memory T cells also express CD127/IL-7Rα.
Gating strategy for observing naive, effector, and memory T cell subsets:
If Propidium Iodide or 7-AAD was used, first gate on viable cells. Next, gate on lymphocytes based on forward scatter and side scatter. Helper T cells are the CD3+CD4+ cells within the lymphocyte gate. Cytotoxic T cells are the CD3+CD8+ cells within the lymphocyte gate. Next, observe expression of CD44 vs. CD62L on either helper T cells or cytotoxic T cells. Naive T cells are CD44-CD62L+. Central memory T cells are CD44+CD62L+. Effector memory and effector cells are CD44+CD62L-. To distinguish effector memory and effector cells, observe expression of CD127/IL-7Rα on the CD44+CD62L- population. Effector memory cells are positive for expression of CD127/IL-7Rα. Effector cells are negative for expression of CD127/IL-7Rα. Naive and central memory T cells also express CD127/IL-7Rα.
Storage
Antibodies are supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. Store at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific “Best By” dates for each individual component.
All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific “Best By” dates for each individual component.
Specificity / Sensitivity
Each antibody in the Mouse Naive/Effector/Memory T Cell Markers Flow Cytometry Panel detects endogenous levels of its target protein and detects epitopes within the extracellular domain. CD3 (17A2) Rat mAb (PE Conjugate) recognizes endogenous levels of total CD3ε, CD3γ, and CD3δ proteins. CD44 (IM7) Rat mAb (PE-Cy5® Conjugate) is expected to detect all isoforms of CD44.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal conditions, with unreacted dye removed from the preparation.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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