Human Natural Killer Cell Markers Flow Cytometry Panel #83586
To Purchase # 83586
Important Ordering Details
Custom Ordering Details: Product is assembled upon order. Please allow up to three business days for your product to be processed.- Product Includes
| Product Includes | Quantity (with Count) | Reactivity | Dilution |
|---|---|---|---|
| CD45 (HI30) Mouse mAb (FITC Conjugate) #86532 | 1 x 500 µl | H | |
| CD3 (UCHT1) Mouse mAb (violetFluor™ 450 Conjugate) #61347 | 1 x 500 µl | H | |
| NCAM1 (CD56) (MY31) Mouse mAb (APC Conjugate) #51997 | 1 x 500 µl | H | |
| CD16 (3G8) Mouse mAb (PE Conjugate) #82004 | 1 x 500 µl | H |
Product Information
Protocol
Product Description
Human natural killer cells are CD45+CD3-CD56+. The large subset with high CD16 expression are mature cytotoxic natural killer cells, while those with low CD16 expression are immature precursors and cytokine producers.
Product Usage Information
Gating strategy for identifying human natural killer cells:
If Propidium Iodide or 7-AAD was used, first gate on viable cells. Next, gate based on CD45 expression, then gate on lymphocytes based on forward scatter and side scatter on CD45+ fraction. NK cells are CD3-CD56+, and these cells can further be analyzed for CD16 expression.
Storage
Antibodies are supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. Store at 4oC. Do not aliquot the antibodies. Protect from light. Do not freeze.
All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific "Best By" dates for each individual component.
Specificity / Sensitivity
Each antibody in the Human Natural Killer Cell Markers Flow Cytometry Panel detects endogenous levels of its target protein and epitopes within the extracellular domains.
Species Reactivity:
Source / Purification
Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal conditions, with unreacted dye removed from the preparation.
限制使用
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