Collagenase, Type 1 #62648

Name: Collagenase, Type 1
Price: 342.00 USD
Availability: InStock

Product Information

Product Usage Information

Collagenase, Type 1 is soluble from 1-20 mg/ml with an optimal pH range of 6.3-7.5. It is recommended to reconstitute with a buffer compatible with the intended assay. Vials should be brought to room temperature prior to opening and they should not be opened in humid areas.

Storage

Collagenase, Type 1 is supplied as a lyophilized powder. This product is stable for 12 months when stored at 4ºC, protected from moisture. It is recommended to reconstitute as needed and to store solutions at a 1 mg/ml concentration at -20ºC or -80ºC. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Collagenase, Type 1 is isolated from Clostridium histolyticum and has the original balance of collagenase, caseinase, clostripain, and tryptic activities. Crude collagenase preparations contain a mixture of several different enzymes, including collagenases and proteases, that effectively break down intercellular matrices. The ratio of collagenolytic and proteolytic activities is an important part of tissue dissociation. Crude collagenase is inhibited by metal chelating agents, such as cysteine, EDTA, or o-phenanthroline, but not DFP. Calcium ions provide structural stability for enzyme activity (1). Collagenases are used at various concentrations for tissue dissociation, generally ranging from 0.1 to 5 mg/ml, and various time points depending on the desired affect (2-4). Several types of collagenases (Types 1, 2, 3, and 4) have been established based on the enzymatic profile.

Activity

Greater than or equal to 125 units per mg dry weight

Unit Definition: One unit releases 1 μmol of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5.

Specificity / Sensitivity

Collagenase, Type 1 is suggested for epithelial, liver, lung, and adrenal primary cell isolations.

Source / Purification

Collagenase, Type 1 is produced by the bacterium Clostridium histolyticum and dialyzed prior to lyophilization.

Background

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit our Trademark Information page.