Render Target: SSR
Render Timestamp: 2024-11-14T22:34:25.327Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-04-05 20:22:21.550
Product last modified at: 2024-06-27T13:36:08.232Z
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PDP - Template Name: Blocking Peptide
PDP - Template ID: *******6db2f4c

Zap-70 Blocking Peptide #1160

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    Product Information

    Product Usage Information

    Use as a blocking reagent to evaluate the specificity of antibody reactivity in Western immunoblotting anddot blot protocols. For western immunoblotting, add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at room temperature for 2 hours before allowing to react with the blot.

    Storage

    Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

    Product Description

    This peptide can be used to block Zap-70 (99F2) Rabbit mAb #2705.

    Quality Control

    The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Zap-70 (99F2) Rabbit mAb #2705 signal completely in both dot blot and Western blotting.

    Background

    The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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