Render Target: SSR
Render Timestamp: 2024-11-14T22:34:21.604Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-20 06:19:58.883
Product last modified at: 2024-10-09T20:00:10.733Z
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PDP - Template Name: IHC Control Slides
PDP - Template ID: *******ff9c92b

SignalSlide® NF-κB p65 IHC Controls #12873

    Product Information

    Product Description

    Each control slide contains formalin fixed, paraffin-embedded HCT 116 cells, both untreated and treated with hTNF-α, that serve as a control for NF-κB p65 immunostaining.

    Background

    Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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