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Render Timestamp: 2025-03-18T11:52:39.530Z
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XML generation date: 2024-09-12 14:38:50.515
Product last modified at: 2024-09-13T07:01:51.972Z
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SHP-1 Phosphatase #8005

Inquiry Info. # 8005

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    Product Information

    Product Description

    Purified recombinant full-length human SHP-1 phosphatase, supplied as a GST fusion protein.
    MW (kDa) 93

    Storage

    Storage:
    Enzyme is supplied in 50 mM Tris-HCl, pH7.5; 150 mM NaCl, 0.25 mM DTT, 0.1mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol, 7 mM glutathione.
    Store at -80° C.
    Keep on ice during use.
    Avoid repeated freeze-thaw cycles.

    Quality Control

    The theoretical molecular weight of the GST-SHP-1 fusion protein is 92 kDa. The purified phosphatase was quality controlled for purity using SDS-PAGE followed by Coomassie stain [Fig.1]. SHP-1 phosphatase activity was determined using a DELFIA® assay [Fig.2].

    Source / Purification

    The GST-phosphatase fusion protein was produced by expressing recombinant human SHP-1 (Met1-Lys597) (GenBank Accession No. NM_080548) with an amino-terminal GST tag in E. coli. The protein was purified by one-step affinity chromatography using glutathione-agarose.

    Background

    SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain (1). SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EPORs) to negatively regulate hematopoietic growth (2). Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation (3). Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R β chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation (4). Because SHP-1 downregulates various proliferation pathways, SHP-1 is considered a potential tumor suppressor and angiogenesis regulator (5,6).
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