Render Target: SSR
Render Timestamp: 2024-12-19T20:58:55.100Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-04-05 20:22:21.474
Product last modified at: 2024-06-27T13:36:08.259Z
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PDP - Template Name: Blocking Peptide
PDP - Template ID: *******6db2f4c

S6 Ribosomal Protein Blocking Peptide #1155

Pricing & Additional Information

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Important Ordering Details

Custom Ordering Details: This product is assembled upon order. Please allow two-four weeks for your product to be processed.

    Product Information

    Product Usage Information

    Use as a blocking reagent to evaluate the specificity of antibody reactivity in Western immunoblotting and dot blot protocols.

    For Western immunoblotting, add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at room

    temperature for 30 minutes before allowing to react with the blot.

    Storage

    Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. S1% DMSO. tore at –20°C.

    Product Description

    This peptide is used to block S6 Ribosomal Protein (5G10) Rabbit mAb # 2217 reactivity.

    Quality Control

    The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks S6 Ribosomal Protein (5G10) Rabbit Monoclonal Antibody # 2217 signal completely in both dot blot and Western blotting.

    Background

    One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).~No product specific background information is currently available for this product.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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