Rb Control Proteins #9303
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Product Information
Product Usage Information
Boil for 3 minutes prior to use. Load 10 μl of phosphorylated and nonphosphorylated Rb Control Proteins per lane.
Storage
Supplied in SDS Sample Buffer: 62.5 mM Tris-HCL (ph 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. Store at -20°C, or at –80°C for long-term storage.
Product Description
Nonphosphorylated Rb-C Fusion Protein (5 µg/ml): Rb-C is expressed as a recombinant fusion protein of Rb residues 701–928 and maltose binding protein serves as a negative control. Supplied in SDS Sample Buffer.
Phosphorylated Rb-C Fusion Protein (5 µg/ml): Rb-C is expressed as a recombinant fusion protein of Rb residues 701–928 and maltose binding protein prepared by in vitro kinase reaction with cdc2 serves as a positive control. Supplied in SDS Sample Buffer.
Note: This truncated Rb recombinant protein is not recognized by Phospho-Rb (Ser608) Antibody #2181, Phospho-Rb (Ser608) (D10F2) Rabbit mAb #8147, Rb (D20) Rabbit mAb #9313, or Phospho-Rb (Thr356) (E3P9O) Rabbit mAb #81403.
Phosphorylated Rb-C Fusion Protein (5 µg/ml): Rb-C is expressed as a recombinant fusion protein of Rb residues 701–928 and maltose binding protein prepared by in vitro kinase reaction with cdc2 serves as a positive control. Supplied in SDS Sample Buffer.
Note: This truncated Rb recombinant protein is not recognized by Phospho-Rb (Ser608) Antibody #2181, Phospho-Rb (Ser608) (D10F2) Rabbit mAb #8147, Rb (D20) Rabbit mAb #9313, or Phospho-Rb (Thr356) (E3P9O) Rabbit mAb #81403.
| MW (kDa) | 76 |
| Molecular Formula | Apparent Molecular Weight: Both the nonphosphorylated and phosphorylated forms of Rb-C migrate at an apparent molecular weight of 76 kDa by SDS-PAGE. |
Background
The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
- Sherr, C.J. (1996) Science 274, 1672-7.
- Nevins, J.R. (1992) Science 258, 424-9.
- Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
- Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
- Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
- Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
- Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
- Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
- Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.
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