Caspase-3 Control Cell Extracts #9663
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- WB
Western blot analysis of Jurkat cell extracts untreated or treated with cytochrome c in vitro, showing full length and/or cleaved caspase-3 (upper) and cleaved caspase-3 Asp175 (lower), using Caspase-3 Antibody #9662 and Cleaved Caspase-3 (Asp175) Antibody #9661.
- Product Includes
- Related Products
| Product Includes | Quantity |
|---|---|
| Caspase-3 Control Cell Extracts (Jurkat untreated) #64514 | 100 µl |
| Caspase-3 Control Cell Extracts (Jurkat +Cytochrome c) #83979 | 100 µl |
Product Information
Product Usage Information
Boil for 3 minutes prior to use. Load 10 ul of untreated and cytochrome c treated Caspase-3 Control Cell Extracts per lane.
Storage
Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol,50 mM DTT, 0.01% w/v bromophenol blue or phenolred.
Store at –20°C, or at –80°C for long-term storage.
Store at –20°C, or at –80°C for long-term storage.
Product Description
Caspase-3 Control Cell Extracts (Jurkat Untreated): Untreated Jurkat cells are lysed in Chaps cell extract buffer and a cytoplasmic fraction is generated to serve as a negative control for caspase cleavage. Supplied in SDS sample buffer.
Caspase-3 Control Cell Extracts (Jurkat +Cytochrome c): Untreated Jurkat cells are lysed in Chaps cell extract buffer and a cytoplasmic fraction is generated. Extracts are treated with cytochrome c in vitro to generate a positive control for caspase cleavage. Supplied in SDS sample buffer.
Caspase-3 Control Cell Extracts (Jurkat +Cytochrome c): Untreated Jurkat cells are lysed in Chaps cell extract buffer and a cytoplasmic fraction is generated. Extracts are treated with cytochrome c in vitro to generate a positive control for caspase cleavage. Supplied in SDS sample buffer.
Background
Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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For Research Use Only. Not For Use In Diagnostic Procedures.
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