PTPN7 Phosphatase #8006
Inquiry Info. # 8006
Please see our recommended alternatives.
Product Information
Product Description
Purified recombinant full-length human PTPN7 phosphatase, supplied as a GST fusion protein.
| MW (kDa) | 66000 |
Storage
Enzyme is supplied in 20 mM MOPS, pH7.5; 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 30% glycerol, 7 mM glutathione.
Store at -80° C.
Keep on ice during use.
Avoid repeated freeze-thaw cycles.
Store at -80° C.
Keep on ice during use.
Avoid repeated freeze-thaw cycles.
Quality Control
The theoretical molecular weight of the GST-PTPN7 fusion protein is 66 kDa. The purified phosphatase was quality controlled for purity using SDS-PAGE followed by Coomassie stain [Fig.1]. PTPN7 phosphatase activity was determined using a DELFIA(R) assay [Fig.2].
Source / Purification
The GST-phosphatase fusion protein was produced by expressing recombinant human PTPN7 (Met1-Pro360) (GenBank Accession No. NM_002832) with an amino-terminal GST tag in E. coli. The protein was purified by one-step affinity chromatography using glutathione-agarose.
Background
Hematopoietic protein tyrosine phosphatase (HePTP, PTPN7) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells (1). The protein is made up of a short, amino-terminal extension that contains a conserved kinase interaction motif (KIM) and a carboxy-terminal protein tyrosine phosphatase domain (1,2). The presence of the kinase interaction motif allows PTPN7 to interact with MAPK family kinases, such as Erk1/2 and p38 MAP kinases (2,3). Reciprocal modification occurs between PTPN7 and Erk2, as the kinase phosphorylates PTPN7 at Thr45 and Ser72 while the phosphatase dephosphorylates Erk2 at Tyr185 (2). Down regulation of MAPK signaling follows removal of specific phospho-tyrosine resides from these MAPK kinases (3,4). Modification of MAPK family kinases by PTPN7 can regulate both kinase activity and localization (4).
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.