渲染靶标：SSR
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  1. Products /
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  3. PathScan® Total SMAD3 Sandwich ELISA Kit

PathScan® Total SMAD3 Sandwich ELISA Kit #12002

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ELISA Image 1: PathScan® Total SMAD3 Sandwich ELISA Kit
Figure 1. Treatment of HeLa cells with hTGF-β3 #8425 stimulates phosphorylation of SMAD3 at Ser423/425, as detected by PathScan® Phospho-SMAD3 (Ser423/425) Sandwich ELISA Kit #12003, but does not affect the level of total SMAD3 protein detected by PathScan® Total SMAD3 Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using SMAD3 (C67H9) Rabbit mAb #9523 (left panel) and a phospho-SMAD3 (Ser423/425) rabbit mAb (right panel) are shown in the bottom figure.
1/2

To Purchase # 12002**

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H M Mi
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Mi-Mink 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
SMAD3 Rabbit mAb Coated Microwells #9545996 tests
SMAD2/3 Mouse Detection mAb #131541 eaGreen (Lyophilized)
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) #133041 eaRed (Lyophilized)
Detection Antibody Diluent #1333911 mlGreen
HRP Diluent #1351511 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
ELISA Sample Diluent #1108325 mlBlue
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The PathScan® Total SMAD3 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that recognizes endogenous levels of SMAD3 protein. A SMAD3 Rabbit Antibody has been coated on the microwells. After incubation with cell lysates, SMAD3 proteins (phospho and nonphospho) are captured by the coated antibody. Following extensive washing, a SMAD3 Mouse Detection Antibody is added to detect captured SMAD3 proteins. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of SMAD3 proteins.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan® Total SMAD3 Sandwich ELISA Kit recognizes endogenous levels of total SMAD3 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse, Mink

Background

Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).

Pathways

Explore pathways related to this product.


Database Links

UniProt ID: P84022


Entrez-Gene Id: 4088


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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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