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  1. Products /
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  3. PathScan® Total ROS1 Sandwich ELISA Kit

PathScan® Total ROS1 Sandwich ELISA Kit #7091

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ELISA Image 1: PathScan® Total ROS1 Sandwich ELISA Kit
Figure 1: Constitutive phosphorylation of ROS1 in HCC78 cells lysed in the presence of phosphatase inhibitors* (phospho lysate) is detected by PathScan® Phospho-ROS1 (panTyr) Sandwich ELISA Kit #7093 (upper, right). In contrast, a low level of phospho-ROS1 protein is detected in HCC78 cells lysed in the absence of phosphatase inhibitors* (nonphospho lysate). Similar levels of total HCC78 protein from both nonphospho and phospho lysates are detected by PathScan® Total ROS1 Sandwich ELISA Kit #7091 (upper, left). Absorbance at 450 nm is shown in the top figure while corresponding western blots using Phospho-ROS1 (Tyr2274) Antibody #3078 (right) or total ROS1 (69D6) Mouse mAb #3266 (left) are shown in the bottom figure.

*Phosphatase inhibitors include sodium pyrophosphate, β-glycerophosphate, and Na3VO4.

1/2

To Purchase # 7091**

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
ROS1 Mouse mAb Coated Microwells #3082896 tests
ROS1 Rabbit Detection mAb #137461 eaGreen (Lyophilized)
Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated) #132721 eaRed (Lyophilized)
Detection Antibody Diluent #1333911 mlGreen
HRP Diluent #1351511 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
ELISA Sample Diluent #1108325 mlBlue
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The PathScan® Total ROS1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of ROS1 protein. A ROS1 mouse antibody has been coated onto the microwells. After incubation with cell lysates, ROS1 protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a ROS1 rabbit detection antibody is added to detect captured ROS1 protein. Anti-rabbit IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of ROS1 protein.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan® Total ROS1 Sandwich ELISA Kit #7091 detects endogenous levels of ROS1 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

ROS1, an orphan receptor tyrosine kinase of the insulin receptor family, was initially identified as a homolog of v-ros from the UR2 sarcoma virus (1). ROS1 consists of a large extracellular domain that is composed of six fibronectin repeats, a transmembrane domain, and a C-terminal kinase domain. Being an orphan receptor, the functions of ROS1 are not well known, though it has been shown to play an important role in differentiation of epididymal epithelium (2). The first oncogenic fusion of ROS1, FIG-ROS1, was initially identified by research studies in glioblastoma (3), and subsequent studies have found this fusion in cholangiocarcinoma (4), ovarian cancer (5), and non-small cell lung cancer (NSCLC) (6). Investigators have found additional oncogenic ROS1 fusion proteins in NSCLC (at a frequency of ~1.6%), where the ROS1 kinase domain is fused to the amino-terminal region of several different proteins, including CD74 and SLC34A2 (6-8). ROS1 fusion proteins activate the SHP-2 phosphatase, PI3K/Akt/mTOR, Erk, and Stat3 pathways (3,4,9). There are two autophosphorylation sites (Tyr2274, Tyr2334) downstream of the kinase domain of ROS1, either of which may serve as biomarkers of ROS1 kinase activity, including that of ROS1 fusion proteins (10).

Pathways

Explore pathways related to this product.


Database Links

UniProt ID: P08922


Entrez-Gene Id: 6098


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PathScan is a registered trademark of Cell Signaling Technology, Inc.
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