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PathScan^® Total Neurofilament-L Sandwich ELISA Kit

PathScan^® Total Neurofilament-L Sandwich ELISA Kit #99175

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Supporting Data

REACTIVITY

M R

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

M-Mouse
R-Rat

Product Information

Product Description

The PathScan^® Total Neurofilament-L Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of neurofilament-L protein. A neurofilament-L mouse mAb has been coated onto the microwells. After incubation with cell lysates, the neurofilament-L proteins are captured by the coated antibody. Following extensive washing, an neurofilament-L rabbit detection mAb is added to detect captured neurofilament-L proteins. Anti-rabbit IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of neurofilament-L protein.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan^® Total Neurofilament-L Sandwich ELISA Kit detects endogenous levels of nerofilament-L protein in mouse or rat brains, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Mouse, Rat

Background

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).

Database Links

UniProt ID: P07196

Entrez-Gene Id: 4747

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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PathScan® Total Neurofilament-L Sandwich ELISA Kit #99175