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PathScan^® Total Ki-67 Sandwich ELISA Kit

PathScan^® Total Ki-67 Sandwich ELISA Kit #14507

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Supporting Data

REACTIVITY

H Mk

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human
Mk-Monkey

Product Information

Product Description

PathScan^® Total Ki-67 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Ki-67. A Ki-67 Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, Ki-67 protein is captured by the coated antibody. Following extensive washing, a Ki-67 Mouse Detection mAb is added to detect the captured Ki-67 protein. An HRP-linked, anti-mouse antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total Ki-67.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan^® Total Ki-67 Sandwich ELISA Kit detects endogenous levels of Ki-67 protein as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Monkey

Background

Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).

Database Links

UniProt ID: P46013

Entrez-Gene Id: 4288

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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PathScan is a registered trademark of Cell Signaling Technology, Inc.

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PathScan® Total Ki-67 Sandwich ELISA Kit #14507