PathScan® Total eIF2α Sandwich ELISA Kit #7952
Filter:
- ELISA
Supporting Data
| REACTIVITY | H |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
The PathScan® Total eIF2α Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of eIF2α protein. A eIF2α rabbit antibody has been coated onto the microwells. After incubation with cell lysates, eIF2α (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a eIF2α mouse antibody is added to detect captured eIF2α protein. Anti-rabbit IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate (TMB) is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total eIF2α protein.
*Antibodies in this kit are custom formulations specific to kit.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
PathScan® Total eIF2α Sandwich ELISA Kit #7952 detects endogenous levels of total eIF2α protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human
Background
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).
- Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
- de Haro, C. et al. (1996) FASEB J. 10, 1378-87.
- Kaufman, R.J. (1999) Genes Dev. 13, 1211-33.
- Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-8.
- Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-6.
- Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-90.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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