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  1. Products /
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  3. PathScan® Total CREB Sandwich ELISA Kit

PathScan® Total CREB Sandwich ELISA Kit #7390

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  • ELISA
ELISA Image 1: PathScan® Total CREB Sandwich ELISA Kit
Figure 1. Treatment of SK-N-MC cells with forskolin stimulates phosphorylation of CREB at Ser133, detected by the PathScan® Phospho-CREB (Ser133) Sandwich ELISA Kit #7385, but does not affect the levels of total CREB detected by PathScan® Total CREB Sandwich ELISA Kit #7390. SK-N-MC cells (80-90% confluent) were treated with 120 μM forskolin with 0.5 mM IBMX for 30 minutes and lysed. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using CREB (48H2) Rabbit mAb #9197 (left panel) or Phospho-CREB (Ser133) (1B6) Mouse mAb #9196 (right panel) are shown in the bottom figure.
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To Purchase # 7390**

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H M R
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
CREB Rabbit mAb Coated Microwells #3592996 tests
CREB Mouse Detection mAb #141041 eaGreen (Lyophilized)
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) #133041 eaRed (Lyophilized)
Detection Antibody Diluent #1333911 mlGreen
HRP Diluent #1351511 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
ELISA Sample Diluent #1108325 mlBlue
PathScan® Sandwich ELISA Lysis Buffer (1X) #701830 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The PathScan® Total CREB Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of CREB. A CREB rabbit antibody has been coated onto the microwells. After incubation with cell lysates, CREB (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a CREB mouse detection antibody is added to detect captured CREB protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total CREB protein.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan® Total CREB Sandwich ELISA Kit #7390 detects endogenous levels of CREB protein as shown in Figure 1. Kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. The kit also cross reacts with the CREB-related protein ATF-1.

Species Reactivity:

Human, Mouse, Rat

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

Pathways

Explore pathways related to this product.


Database Links

UniProt ID: P16220


Entrez-Gene Id: 1385


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PathScan is a registered trademark of Cell Signaling Technology, Inc.
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