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PathScan® Total α-Synuclein Sandwich ELISA Kit #54269

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  • ELISA
ELISA Image 1: PathScan® Total α-Synuclein Sandwich ELISA Kit
Figure 1: α-Synuclein protein is detected in mouse or rat brain tissues using the PathScan® Total α-Synuclein Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using α-Synuclein (D37A6) XP® Rabbit mAb #4179 is shown in the bottom figure.

To Purchase # 54269**

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY M R
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • M-Mouse 
  • R-Rat 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
α-Synuclein Rabbit mAb Coated Microwells #2413996 tests
α-Synuclein Mouse Detection mAb #984151 eaGreen (Lyophilized)
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) #133041 eaRed (Lyophilized)
Detection Antibody Diluent #1333911 mlGreen
HRP Diluent #1351511 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
ELISA Wash Buffer (20X) #980110 ml
Sealing Tape #545032 ea
ELISA Sample Diluent #1108325 mlBlue
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The PathScan® Total α-Synuclein Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of
α-synuclein protein. An α-synuclein rabbit mAb has been coated onto the microwells. After incubation with cell lysates, the α-synuclein protein is captured by the coated antibody. Following extensive washing, α-synuclein mouse detection mAb is added to detect captured α-synuclein protein. Anti-mouse, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of α-synuclein protein.

*Antibodies in this kit are custom formulations specific to the kit.

Protocol

Specificity / Sensitivity

PathScan® Total α-Synuclein Sandwich ELISA Kit detects endogenous levels of α-synuclein protein in mouse or rat tissues, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Mouse, Rat

Background

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-Synuclein gene are linked to Parkinson's disease (1).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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