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PathScan® Total α-Synuclein Sandwich ELISA Kit #54269

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  • ELISA

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

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    Supporting Data

    REACTIVITY M R
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Description

    The PathScan® Total α-Synuclein Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of
    α-synuclein protein. An α-synuclein rabbit mAb has been coated onto the microwells. After incubation with cell lysates, the α-synuclein protein is captured by the coated antibody. Following extensive washing, α-synuclein mouse detection mAb is added to detect captured α-synuclein protein. Anti-mouse, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of α-synuclein protein.

    *Antibodies in this kit are custom formulations specific to the kit.

    Protocol

    Specificity / Sensitivity

    PathScan® Total α-Synuclein Sandwich ELISA Kit detects endogenous levels of α-synuclein protein in mouse or rat tissues, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Mouse, Rat

    Background

    α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-Synuclein gene are linked to Parkinson's disease (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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