PathScan® Total α-Synuclein Sandwich ELISA Kit #54269
Filter:
- ELISA
Supporting Data
| REACTIVITY | M R |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
Product Information
Product Description
The PathScan® Total α-Synuclein Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of
α-synuclein protein. An α-synuclein rabbit mAb has been coated onto the microwells. After incubation with cell lysates, the α-synuclein protein is captured by the coated antibody. Following extensive washing, α-synuclein mouse detection mAb is added to detect captured α-synuclein protein. Anti-mouse, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of α-synuclein protein.
*Antibodies in this kit are custom formulations specific to the kit.
α-synuclein protein. An α-synuclein rabbit mAb has been coated onto the microwells. After incubation with cell lysates, the α-synuclein protein is captured by the coated antibody. Following extensive washing, α-synuclein mouse detection mAb is added to detect captured α-synuclein protein. Anti-mouse, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of α-synuclein protein.
*Antibodies in this kit are custom formulations specific to the kit.
Protocol
Specificity / Sensitivity
PathScan® Total α-Synuclein Sandwich ELISA Kit detects endogenous levels of α-synuclein protein in mouse or rat tissues, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Mouse, Rat
Background
α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-Synuclein gene are linked to Parkinson's disease (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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