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PathScan® RP Total Stat2 Sandwich ELISA Kit #87868

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ELISA Image 1: PathScan® RP Total Stat2 Sandwich ELISA Kit
Figure 1. Treatment of U266B1 cells with Human Interferon-α1 (hIFN-α1) stimulates phosphorylation of Stat2 at Tyr690, but does not affect the level of total Stat2. The relationship between lysate protein concentration from untreated and hIFN-α1-treated U266B1 cells and the absorbance at 450 nm using the PathScan® RP Total Stat2 Sandwich ELISA Kit #87868 is shown in the upper figure. The corresponding western blots using phospho-Stat2 (Tyr690) antibody (left panel) and Stat2 antibody (right panel) are shown in the lower figure. Serum starved U266B1 cells were treated with hIFN-α1 (10 ng/mL) for 15 min at 37°C and then lysed.
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To Purchase # 87868**

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
Stat2 Mouse mAb Coated Microwells #8065596 tests
Stat2 Rabbit Detection mAb #405891 eaRed (Lyophilized)
HRP Diluent #135155.5 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The rapid protocol (RP) PathScan® RP Total Stat2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Stat2 in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with Stat2 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of Stat2. Learn more about your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The PathScan® RP Total Stat2 Sandwich ELISA Kit detects endogenous levels of Stat2. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

Stat2 (113 kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via associations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transcription from the ISRE (5).

Database Links

UniProt ID: P52630


Entrez-Gene Id: 6773


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