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PathScan^® RP Total RSK1 Sandwich ELISA Kit

PathScan^® RP Total RSK1 Sandwich ELISA Kit #7966

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Supporting Data

REACTIVITY

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

The rapid protocol (RP) PathScan^® RP Total RSK1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Total RSK1 protein in a reduced assay time of 1.5 hours. Incubation of cell lysate and detection antibody on the coated microwell plate forms a sandwich with Total RSK1 protein in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of the absorbance for the developed color is proportional to the quantity of Total RSK1 protein. Learn more about all of your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to the kit.

Protocol

Specificity / Sensitivity

The PathScan^® RP Total RSK1 Sandwich ELISA Kit #7966 detects endogenous levels of total RSK1 protein. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

Database Links

UniProt ID: Q15418

Entrez-Gene Id: 6195

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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PathScan® RP Total RSK1 Sandwich ELISA Kit #7966