PathScan® RP Total IRF-7 Sandwich ELISA Kit #45011
Filter:
- ELISA
Supporting Data
| REACTIVITY | M |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
The rapid protocol (RP) PathScan® RP Total IRF-7 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of IRF-7 protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with IRF-7 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of IRF-7 protein. Learn more about your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
The PathScan® RP Total IRF-7 Sandwich ELISA Kit detects endogenous levels of IRF-7 protein. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Mouse
Background
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).
IRF-7, which is functionally similar to IRF-3, is preferentially expressed in lymphoid cells and induced by virus, LPS, and IFN-α (3-5). IRF-7 plays an essential role in the induction of type I interferon in response to viral infection (6-8). IRF-7 is regulated at multiple serine phosphorylation sites near the carboxyl terminus, similar to IRF-3, which are required for nuclear translocation, DNA binding, and transcriptional activity (9-11).
IRF-7, which is functionally similar to IRF-3, is preferentially expressed in lymphoid cells and induced by virus, LPS, and IFN-α (3-5). IRF-7 plays an essential role in the induction of type I interferon in response to viral infection (6-8). IRF-7 is regulated at multiple serine phosphorylation sites near the carboxyl terminus, similar to IRF-3, which are required for nuclear translocation, DNA binding, and transcriptional activity (9-11).
- Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
- Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
- Au, W.C. et al. (1998) J Biol Chem 273, 29210-7.
- Wathelet, M.G. et al. (1998) Mol Cell 1, 507-18.
- Marié, I. et al. (1998) EMBO J 17, 6660-9.
- Sato, M. et al. (2000) Immunity 13, 539-48.
- Honda, K. et al. (2005) Nature 434, 772-7.
- Colina, R. et al. (2008) Nature 452, 323-8.
- Lin, R. et al. (2000) J Biol Chem 275, 34320-7.
- Yang, H. et al. (2003) J Biol Chem 278, 15495-504.
- Caillaud, A. et al. (2005) J Biol Chem 280, 17671-7.
限制使用
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