PathScan® RP SARS-CoV-2 Nucleocapsid Protein Sandwich ELISA Kit #37393
Filter:
- ELISA
Supporting Data
| REACTIVITY | Vir |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- Vir-Virus
Product Information
Product Description
The rapid protocol (RP) PathScan® RP SARS-CoV-2 Nucleocapsid Protein Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of SARS-CoV-2 nucleocapsid protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with SARS-CoV-2 nucleocapsid protein in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of SARS-CoV-2 nucleocapsid protein. Learn more about all of your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
The PathScan® RP SARS-CoV-2 Nucleocapsid Protein Sandwich ELISA Kit detects endogenous levels of SARS-CoV-2 nucleocapsid protein, but does not cross-react with nucleocapsid proteins from SARS or MERS coronaviruses, as shown in Figures 1 and 2. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Virus
Background
The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). The nucleocapsid (N) protein is a phosphoprotein that plays a critical role in viral assembly (3,4). It binds to the viral genomic RNA, enabling its packaging into the ribonucleoprotein complex (RNP), and is the most abundant protein in the virion (5). Evidence from studies in SARS-CoV-2 and other coronaviruses suggest functions for nucleocapsid (N) protein beyond viral RNA packaging, including roles in viral replication (6) and suppressing host immune responses to viral infection (7).
- Zhou, P. et al. (2020) Nature 579, 270-273.
- Tortorici, M.A. and Veesler, D. (2019) Adv Virus Res 105, 93-116.
- Zengel, J. et al. (2015) J Virol 89, 7338-47.
- Surjit, M. et al. (2005) J Virol 79, 11476-86.
- Peng, Y. et al. (2020) EMBO J, e105938.
- Cong, Y. et al. (2020) J Virol 94, e01925-19. doi: 10.1128/JVI.01925-19.
- Mu, J. et al. (2020) Sci China Life Sci 63, 1-4.
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