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  1. Products /
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  3. PathScan® RP Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit

PathScan® RP Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit #7335

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ELISA Image 1: PathScan® RP Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit
Figure 1: Linear relationship between protein concentration of lysates from PAE/CKR cells expressing a chimeric CSF-1R/VEGFR2 receptor and kit assay optical density readings, as detected by PathScan® RP Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA kit #7335. OD450 readings are shown in the top figure, while the corresponding western blot using Phospho-VEGFR-2 (Tyr1175) Rabbit mAb (right panel) or VEGF Receptor 2 (55B11) Rabbit mAb (left panel), is shown in the bottom figure. PAE/CKR cells (85-90% confluent) were treated with 50ng/ml CSF1 for 5 minutes after overnight serum starvation, and then lysed. PAE/CKR cells overexpress chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor 2 intracellular domains (Rahimi, N. et al. [2000] J. Biol. Chem. 275, 16986-16992).
1/2

To Purchase # 7335**

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H M
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
VEGF Receptor 2 Rabbit mAb Coated Microwells #4114196 tests
Phospho-VEGF Receptor 2 (Tyr1175) Rabbit Detection mAb #137591 eaRed (Lyophilized)
HRP Diluent #135155.5 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The rapid protocol PathScan® RP Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Phospho-VEGFR-2 (Tyr1175) protein in a reduced assay time of 1.5 hours.

Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with VEGFR2 protein phosphorylated at Tyr1175 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of VEGFR2 protein phosphorylated at Tyr1175. Learn more about all of your ELISA kit options here.



*Antibodies in kit are custom formulations specific to kit.


Protocol

Specificity / Sensitivity

The PathScan® RP Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit detects endogenous levels of VEGFR2 protein phosphorylated at Tyr1175. The kit sensitivity is shown in Figure 1 (mouse) and Figure 2 (human). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse

Background

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

Pathways

Explore pathways related to this product.


Database Links

UniProt ID: P35968


Entrez-Gene Id: 3791


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PathScan is a registered trademark of Cell Signaling Technology, Inc.
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