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  1. Products /
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  3. PathScan® RP Phospho-Bcl-2 (Ser70) Sandwich ELISA Kit

PathScan® RP Phospho-Bcl-2 (Ser70) Sandwich ELISA Kit #90331

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  • ELISA

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

    Supporting Data

    REACTIVITY H
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    The rapid protocol (RP) PathScan® RP Phospho-Bcl-2 (Ser70) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Bcl-2 protein phosphorylated at Ser70 in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-Bcl-2 (Ser70) in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of phospho-Bcl-2 (Ser70). Learn more about your ELISA kit options here.

    *Antibodies in this kit are custom formulations specific to kit.

    Specificity / Sensitivity

    The PathScan® RP Phospho-Bcl-2 (Ser70) Sandwich ELISA Kit detects endogenous levels of Bcl-2 protein phosphorylated at Ser70. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human

    Background

    Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

    Database Links

    UniProt ID: P10415


    Entrez-Gene Id: 596


    限制使用

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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    PathScan is a registered trademark of Cell Signaling Technology, Inc.
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