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PathScan^® RP Phospho-BCKDH-E1α (Ser293) Sandwich ELISA Kit

PathScan^® RP Phospho-BCKDH-E1α (Ser293) Sandwich ELISA Kit #36390

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Supporting Data

REACTIVITY

H M R

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Description

The rapid protocol (RP) PathScan^® RP Phospho-BCKDH-E1α (Ser293) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of BCKDH-E1α protein phosphorylated at Ser293 in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-BCKDH-E1α (Ser293) in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of phospho-BCKDH-E1α (Ser293). Learn more about your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The PathScan^® RP Phospho-BCKDH-E1α (Ser293) Sandwich ELISA Kit detects endogenous levels of BCKDH-E1α protein phosphorylated at Ser293. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse, Rat

Background

Branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascular and metabolic disorders (1). The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway (2,3). Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex (3). The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regulation of BCKDH. Phosphorylation of BCKDH-E1α was shown to play a key role in regulating the enzymatic activity of this complex (3-5).
Phosphorylation of BCKDH-E1α at Ser293 inactivates BCKDH (3,4). A significant elevation in plasma BCAA levels was reported to correlate with increased phosphorylation of BCKDH-E1α at Ser293 and suppressed BCKDH activity in the liver of diabetic mice (5).

Database Links

UniProt ID: P12694

Entrez-Gene Id: 593

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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PathScan® RP Phospho-BCKDH-E1α (Ser293) Sandwich ELISA Kit #36390