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PathScan^® RP G4S Linker/CD3ζ CAR Sandwich ELISA Kit

PathScan^® RP G4S Linker/CD3ζ CAR Sandwich ELISA Kit #20418

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ELISA

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Supporting Data

REACTIVITY

All

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Description

The rapid protocol (RP) PathScan^® RP G4S Linker/CD3ζ CAR Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects scFv-based chimeric antigen receptors (CARs) containing a G4S linker and a CD3ζ signaling domain in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with CARs containing a G4S linker in a single step by using one antibody that recognizes CD3ζ and another antibody that recognizes the G4S linker. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of scFv-based CARs containing a G4S linker and a CD3ζ signaling domain. Learn more about your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The PathScan^® RP G4S Linker/CD3ζ CAR Sandwich ELISA Kit detects exogenously expressed levels of scFv-based CARs containing a G4S linker and a CD3ζ signaling domain by using one antibody that recognizes CD3ζ and another antibody that recognizes the G4S linker. The kit sensitivity is shown in Figure 1.

Species Reactivity:

All Species Expected

Background

The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)₃ or20-mer(G4S)₄within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2).

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

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PathScan® RP G4S Linker/CD3ζ CAR Sandwich ELISA Kit #20418