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  1. Products /
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  3. PathScan® RP EGF Receptor (E746-A750del Specific) Sandwich ELISA Kit

PathScan® RP EGF Receptor (E746-A750del Specific) Sandwich ELISA Kit #34699

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ELISA Image 1: PathScan® RP EGF Receptor (E746-A750del Specific) Sandwich ELISA Kit
Figure 1. HCC827 cells express EGFR (E746-A750del) mutant protein, whereas NCI-H3255 cells express EGFR (L858R) mutant protein, A-431 cells express wild-type EGFR, and MCF7 cells do not express EGFR. The absorbance readings at 450 nm for a panel of cell extracts (0.1 mg/mL) using the PathScan® RP EGF Receptor (E746-A750del Specific) Sandwich ELISA Kit #34699 are shown in the upper figure. The corresponding western blots using EGFR antibody, EGFR (L858R Mutant Specific) antibody, and EGFR (E746-A750del Specific) antibody are shown in the lower figure.
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To Purchase # 34699**

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
EGF Receptor (E746-A750del Specific) Rabbit mAb Coated Microwells #5197596 tests
EGF Receptor Rabbit Detection mAb #718281 eaRed (Lyophilized)
HRP Diluent #135155.5 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The rapid protocol (RP) PathScan® RP EGF Receptor (E746-A750del Specific) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of EGFR (E746-A750del) mutant protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with EGFR (E746-A750del) mutant protein in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of EGFR (E746-A750del) mutant protein. Learn more about all of your ELISA kit options here.

​​​​​​​*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The PathScan® RP EGF Receptor (E746-A750del Specific) Sandwich ELISA Kit detects endogenous levels of EGFR (E746-A750del) mutant protein, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarcinomas that respond to EGFR inhibitors, such as gefitinib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.

Pathways

Explore pathways related to this product.


Database Links

UniProt ID: P00533


Entrez-Gene Id: 1956


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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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