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  1. Products /
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  3. PathScan® RP Cleaved Gasdermin D (Asp275) Chemiluminescent Sandwich ELISA Kit

PathScan® RP Cleaved Gasdermin D (Asp275) Chemiluminescent Sandwich ELISA Kit #65990

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ELISA Image 1: PathScan® RP Cleaved Gasdermin D (Asp275) Chemiluminescent Sandwich ELISA Kit
Figure 1. The relationship between lysate protein concentration from 293T cells, either mock transfected or transiently transfected with an expression construct encoding Myc/DDK-tagged cleaved Gasdermin D (Asp275), and the immediate light generation with chemiluminescent substrate using the PathScan® RP Cleaved Gasdermin D (Asp275) Chemiluminescent Sandwich ELISA Kit #65990 is shown in the upper figure. The corresponding western blot using cleaved Gasdermin D (Asp275) antibody is shown in the lower figure.
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To Purchase # 65990**

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
Gasdermin D Rabbit mAb Coated Microwells #4491996 tests
Cleaved Gasdermin D (Asp275) Rabbit Detection mAb #397151 eaRed (Lyophilized)
HRP Diluent #135155.5 mlRed
Luminol/Enhancer Solution #848503 ml
Stable Peroxide Buffer #425523 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The rapid protocol (RP) PathScan® RP Cleaved Gasdermin D (Asp275) Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Gasdermin D protein when cleaved at Asp275 in a reduced assay time of 1.5 hours. This chemiluminescent ELISA, which is offered in low volume microplates, shows increased signal and sensitivity while using a smaller sample size. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with cleaved Gasdermin D (Asp275) in a single step. The plate is then extensively washed and chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of cleaved Gasdermin D (Asp275). Learn more about all of your ELISA kit options here.

​​​​​​​*Antibodies in this kit are custom formulations specific to kit
​​​​​​​

Protocol

Specificity / Sensitivity

The PathScan® RP Cleaved Gasdermin D (Asp275) Chemiluminescent Sandwich ELISA Kit detects endogenous levels of Gasdermin D protein only when cleaved at Asp275. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).

Database Links

UniProt ID: P57764


Entrez-Gene Id: 79792


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