PathScan® RP CasMINI Sandwich ELISA Kit #63975
Supporting Data
| REACTIVITY | All |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
The rapid protocol (RP) PathScan® RP CasMINI Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of CasMINI protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with CasMINI protein in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of CasMINI protein. Learn more about your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1/Cas12a (CRISPR from Prevotella and Francisella) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1/Cas12a and Class 2 CRISPR endonucleases permit efficient and specific genome editing that may be used as gene therapies for genetic diseases. However, virus-based vehicles used for in vivo delivery, like adeno-associated virus (AAV)-based vectors, have a limited packaging capacity. CasMINI was engineered from the naturally occurring type V-F Cas12f (Cas14) system. CasMINI is 529 amino acids, which is significantly smaller than Cpf1/Cas12a and Cas9 proteins (62% and 57% smaller, respectively), making CasMINI more suitable for virus-based packaging for in vivo delivery (4).
限制使用
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