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PathScan^® RP BDNF Sandwich ELISA Kit

PathScan^® RP BDNF Sandwich ELISA Kit #94097

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ELISA

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Supporting Data

REACTIVITY

H M

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Description

The rapid protocol (RP) PathScan^® RP BDNF Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of BDNF protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with BDNF in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of BDNF. Learn more about your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The PathScan^® RP BDNF Sandwich ELISA Kit detects both full-length BDNF protein and the mature form of BDNF. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse

Background

Neurotrophins are composed of at least four family members, including NGF, BDNF, NT-3, and NT-4, and all are known to influence growth, development, differentiation, and survival of neurons (1). Proneurotrophins bind to p75NTR, but not to the family of Trk receptor tyrosine kinases (Trk), and following maturation, BDNF binds and activates TrkB. Trk receptors, in turn, activate three major signaling pathways: (a) Ras-MAPK signaling, which promotes neuronal differentiation and neurite outgrowth, (b) PI3K/Akt signaling, which promotes survival and growth of neurons, and (c) PLC-γ1-PKC signaling, which promotes synaptic plasticity (2). BDNF is a major regulator of transmission and plasticity at adult synapses. Moreover, the precursor proBDNF and the mature protein mBDNF drive opposite effects on long-term potentiation and long-term depression (3). BDNF has also been implicated in body weight regulation and activity: heterozygous BDNF knockout mice are hyperphagic, obese, and hyperactive (4).

Database Links

UniProt ID: P23560

Entrez-Gene Id: 627

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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PathScan is a registered trademark of Cell Signaling Technology, Inc.

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PathScan® RP BDNF Sandwich ELISA Kit #94097