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PathScan^® Phospho-Stat1 (Tyr701) Sandwich ELISA Kit

PathScan^® Phospho-Stat1 (Tyr701) Sandwich ELISA Kit #73582

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Supporting Data

REACTIVITY

H M

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Description

The PathScan^® Phospho-Stat1 (Tyr701) Sandwich ELISA Kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Stat1 protein phosphorylated at Tyr701. A Stat1 rabbit antibody has been coated onto the microwells. After incubation with cell lysates, Stat1 protein (phosphorylated and nonphosphorylated) is captured by the coated antibody. Following extensive washing, a Phospho-Stat1 (Tyr701) mouse detection antibody is added to detect the captured phospho-Stat1 (Tyr701) protein. HRP-linked anti-mouse antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of Stat1 protein phosphorylated at Tyr701.

*Antibodies in this kit are custom formulations specific to the kit.

Protocol

Specificity / Sensitivity

The PathScan^® Phospho-Stat1 (Tyr701) Sandwich ELISA Kit detects endogenous levels of Stat1 protein phosphorylated at Tyr701. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse

Background

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

Database Links

UniProt ID: P42224

Entrez-Gene Id: 6772

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

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PathScan® Phospho-Stat1 (Tyr701) Sandwich ELISA Kit #73582