Name: FastScan™ Phospho-IκBα (Ser32/36) ELISA Kit
Price: 625.00 USD
Availability: InStock

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ELISA

Important Ordering Details

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Supporting Data

REACTIVITY

H M R

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Description

The FastScan™ Phospho-IκBα (Ser32/36) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of IκBα when phosphorylated at Ser32/36. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-IκBα (Ser32/36) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-IκBα (Ser32/36).

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The FastScan™ Phospho-IκBα (Ser32/36) ELISA Kit detects endogenous levels of IκBα when phosphorylated at Ser32/36, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse, Rat

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

Database Links

UniProt ID: P25963

Entrez-Gene Id: 4792

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.

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FastScan™ Phospho-IκBα (Ser32/36) ELISA Kit #92035