FastScan™ Citrullinated Histone H3 (Arg2) ELISA Kit #95294
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- ELISA
Important Ordering Details
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Supporting Data
| REACTIVITY | H |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
The FastScan™ Citrullinated Histone H3 (Arg2) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of histone H3 citrullinated at Arg2. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with citrullinated histone H3 (Arg2) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of citrullinated histone H3 (Arg2).
*Antibodies in this kit are custom formulations specific to kit.
IMPORTANT: This FastScan™ ELISA Kit requires 4 washes at Step 6 of the protocol.
*Antibodies in this kit are custom formulations specific to kit.
IMPORTANT: This FastScan™ ELISA Kit requires 4 washes at Step 6 of the protocol.
Protocol
Specificity / Sensitivity
The FastScan™ Citrullinated Histone H3 (Arg2) ELISA Kit detects endogenous levels of histone H3 citrullinated at Arg2, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human
Background
The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Histone post-translational modifications, such as methylation, acetylation, ubiquitination, and citrullination, influence chromatin accessibility and regulate gene expression (1). Histone citrullination is carried out by protein arginine deiminases (PADs), which convert positively charged arginine residues into neutral citrulline (2). Histone citrullination affects the binding of histone reader proteins to chromatin, and it is associated with both transcriptional activation and repression (3-5). Histone citrullination is also a major marker for NETosis, which is a programmed cell death mechanism carried out by neutrophils (6). Deregulation of NETosis is associated with multiple autoimmune diseases, including rheumatoid arthritis (RA) and lupus (7,8). Histone hypercitrullination via PAD4 may also play a role in the progression of various cancers, including multiple myeloma (MM), acute myeloid leukemia (AML), lung cancer, and hepatocellular carcinoma (HCC) (9-12).
- Shanmugam, M.K. et al. (2018) Oncotarget 9, 11414-11426.
- Fuhrmann, J. and Thompson, P.R. (2016) ACS Chem Biol 11, 654-68.
- Wang, Y. et al. (2004) Science 306, 279-83.
- Cuthbert, G.L. et al. (2004) Cell 118, 545-53.
- Zhang, X. et al. (2012) Proc Natl Acad Sci USA 109, 13331-6.
- Hamam, H.J. and Palaniyar, N. (2019) Biomolecules 9, 369. doi: 10.3390/biom9080369.
- Corsiero, E. et al. (2016) Front Immunol 7, 485.
- Hakkim, A. et al. (2010) Proc Natl Acad Sci U S A 107, 9813-8.
- McNee, G. et al. (2017) Leukemia 31, 373-381.
- Song, G. et al. (2016) Oncotarget 7, 3144-57.
- Tanikawa, C. et al. (2012) Nat Commun 3, 676.
- Lu, M. et al. (2020) J Med Virol 92, 1221-1230.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.
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