FastScan™ Citrullinated Histone H3 (Arg17) ELISA Kit #82818
- ELISA
Supporting Data
REACTIVITY | H |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
The FastScan™ Citrullinated Histone H3 (Arg17) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of histone H3 when citrullinated at Arg17. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with citrullinated histone H3 (Arg17) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of citrullinated histone H3 (Arg17).
*Antibodies in kit are custom formulations specific to kit.
IMPORTANT: This FastScan™ ELISA Kit requires 4 washes at Step 6 of the protocol.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Shanmugam, M.K. et al. (2018) Oncotarget 9, 11414-11426.
- Fuhrmann, J. and Thompson, P.R. (2016) ACS Chem Biol 11, 654-68.
- Wang, Y. et al. (2004) Science 306, 279-83.
- Cuthbert, G.L. et al. (2004) Cell 118, 545-53.
- Zhang, X. et al. (2012) Proc Natl Acad Sci USA 109, 13331-6.
- Hamam, H.J. and Palaniyar, N. (2019) Biomolecules 9, 369. doi: 10.3390/biom9080369.
- Corsiero, E. et al. (2016) Front Immunol 7, 485.
- Hakkim, A. et al. (2010) Proc Natl Acad Sci U S A 107, 9813-8.
- McNee, G. et al. (2017) Leukemia 31, 373-381.
- Song, G. et al. (2016) Oncotarget 7, 3144-57.
- Tanikawa, C. et al. (2012) Nat Commun 3, 676.
- Lu, M. et al. (2020) J Med Virol 92, 1221-1230.
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