Render Target: SSR
Render Timestamp: 2025-01-02T13:09:29.309Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-12-25 01:37:21.973
Product last modified at: 2025-01-01T09:01:26.947Z
1% for the planet logo
PDP - Template Name: FastScan ELISA Kit
PDP - Template ID: *******a26362b

FastScan Citrullinated Histone H3 (Arg17) ELISA Kit #82818

Filter:
  • ELISA
ELISA Image 1: FastScan™ Citrullinated Histone H3 (Arg17) ELISA Kit
Figure 1. Citrullination of histone H3 is induced by overexpression of PADI4, in combination with activation by Locke’s Solution and A23187 ionophore treatment. The relationship between lysate protein concentration from cell extracts and the absorbance at 450 nm using the FastScan™ Citrullinated Histone H3 (Arg17) ELISA Kit #82818 is shown in the upper figure. The corresponding western blots using citrullinated histone H3 (Arg17) antibody (left panel) and β-Actin antibody (right panel) are shown in the lower figure. 293T cells were mock transfected or transfected with a construct expressing human PADI4 (hPADI4), and untreated or treated as indicated with: Locke’s Solution (30 min) and A23187 ionophore (5 µM, 30 min) in Locke’s Solution, and then lysed.

To Purchase # 82818C**

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolume (with Count)Solution Color
FastScan™ ELISA Microwell Strip Plate, 96 Well #532571 x 96 tests
Histone H3 Rabbit Capture mAb #106061 x 1 eaGreen (Lyophilized)
Citrullinated Histone H3 (Arg17) Rabbit HRP-linked mAb #289671 x 1 eaRed (Lyophilized)
FastScan™ ELISA Capture Antibody Diluent #160761 x 3 mlGreen
FastScan™ ELISA HRP Antibody Diluent #281201 x 3 ml
TMB Substrate #70041 x 11 ml
STOP Solution #70021 x 11 ml
Sealing Tape #545031 x 1 ea
ELISA Wash Buffer (20X) #98011 x 25 ml
FastScan™ ELISA Cell Extraction Buffer (5X) #699051 x 10 ml
FastScan™ ELISA Cell Extraction Enhancer Solution (50X) #252431 x 1 ml
FastScan™ ELISA Kit #82818 Positive Control Type 2 #963131 x 1 ea

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The FastScan™ Citrullinated Histone H3 (Arg17) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of histone H3 when citrullinated at Arg17. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with citrullinated histone H3 (Arg17) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of citrullinated histone H3 (Arg17).

*Antibodies in kit are custom formulations specific to kit.

IMPORTANT: This FastScan™ ELISA Kit requires 4 washes at Step 6 of the protocol.

Protocol

Specificity / Sensitivity

The FastScan™ Citrullinated Histone H3 (Arg17) ELISA Kit detects endogenous levels of histone H3 when citrullinated at Arg17, as shown in Figure 1. This kit may also recognize histone H3 citrullinated at residue Arg26. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Histone post-translational modifications, such as methylation, acetylation, ubiquitination, and citrullination, influence chromatin accessibility and regulate gene expression (1). Histone citrullination is carried out by protein arginine deiminases (PADs), which convert positively charged arginine residues into neutral citrulline (2). Histone citrullination affects the binding of histone reader proteins to chromatin, and it is associated with both transcriptional activation and repression (3-5). Histone citrullination is also a major marker for NETosis, which is a programmed cell death mechanism carried out by neutrophils (6). Deregulation of NETosis is associated with multiple autoimmune diseases, including rheumatoid arthritis (RA) and lupus (7,8). Histone hypercitrullination via PAD4 may also play a role in the progression of various cancers, including multiple myeloma (MM), acute myeloid leukemia (AML), lung cancer, and hepatocellular carcinoma (HCC) (9-12).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.