Interleukin-4 (IL-4) #3836
Western blot analysis of extracts from ACHN cells treated with various concentrations of Human Interleukin-4 (IL-4) for 15 minutes using Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb #9364 (upper) and total Stat6 Antibody #9362 (lower).
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Product Information
Product Usage Information
The working concentration generally ranges from 10-50 ng/ml.
Storage
Lyophilized product is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.
Product Description
Recombinant human IL-4 was prepared in E. coli and and is supplied in a lyophilized form. A greater than 98% purity was determined by reverse phase-HPLC and SDS-PAGE.
| MW (kDa) | 15 |
| Purity | >95% |
| Activity | The specific activity is 5 X 106 IU/mg. |
Source / Purification
Recombinant human IL-4 expressed in E. coli
Background
Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).
- Yokota, T. et al. (1986) Proc Natl Acad Sci USA 83, 5894-8.
- Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.
- Kopf, M. et al. (1993) Nature 362, 245-8.
- Kotowicz, K. and Callard, R.E. (1993) Eur J Immunol 23, 2250-6.
- Thyphronitis, G. et al. (1989) Proc Natl Acad Sci USA 86, 5580-4.
- Hou, J. et al. (1994) Science 265, 1701-6.
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